Use of recA gene sequence analysis for the identification of 3 Staphylococcus equorum strains predominant on dry - cured 4 hams 5 6 7
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Characterization of coagulase-negative staphylococci isolated from Spanish dry cured meat products.
Technological and safety-related properties were analyzed in a coagulase-negative staphylococci (CNS) collection isolated from Spanish dry-cured meat products in order to use them as starter cultures. The highest nitrate reductase and proteolytic activity was showed by Staphylococcus carnosus and Staphylococcus equorum. Only a few strains were able to form biofilms and the presence of the ica g...
متن کاملEnterotoxigenicity of Staphylococcus strains isolated from Spanish dry-cured hams.
The ability of 135 Staphylococcus strains isolated from Spanish dry-cured hams to produce enterotoxins in culture was investigated by the reversed passive latex agglutination method. A high percentage of enterotoxigenic Staphylococcus aureus strains (85.9%) was recorded, and 54.3% of these produced enterotoxin A. One of the two Staphylococcus epidermidis strains produced enterotoxin C. The reve...
متن کاملIdentification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers
For the first time in Iran 40 strains of Bifidobacterium were isolated from feces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as Bifidobacterium longum, 10 as Bifidobacterium bifidum and one as Bifidobacterium catenolatum. In order to validate these results and also to identify other isolates that had not been identified by phenotypic tests, two methods of PCR wi...
متن کاملMolecular Identification of Rare Clinical Mycobacteria by Application of 16S-23S Spacer Region Sequencing
Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical ...
متن کاملBiodiversity of coagulase-negative Staphylococci in French cheeses, dry fermented sausages, processing environments and clinical samples.
In this study, the biodiversity of Coagulase-Negative Staphylococci (CNS) strains isolated in France from cheese related samples (227 isolates) and dry sausage related samples (204 isolates) was compared to the biodiversity of 297 clinical isolates. Species identification was performed using different molecular methods (specific PCR, "Staph array" hybridization and sodA gene sequencing). Infras...
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